Fig. 4. Representative photographs of renal tissues sections stained with Sirus Red from control rats (A), and rats treated with adenine (B and C), canagliflozin 10 mg/kg (D), canagliflozin 10 mg/kg + adenine (E and F), canagliflozin 25 mg/kg (G), and canagliflozin 25 mg/kg + adenine (H and I). No evidence of interstitial fibrosis was seen in the control or canagliflozin groups (A, D, and G). Adenine feeding induced significant interstitial fibrosis (B and C). The two groups that received canagliflozin + adenine showed significant improvement in the interstitial fibrosis (E, F, H and I).